Extracellular Enzyme Activity of Yeast Isolates from Gringsingan Flowers (Hyptis Suaveolens (L.) Poit) in Kupang as Extracellular Enzyme Producers

Abstract

Yeast can be found in flowers because flowers contain nectar which is a source of nutrition for yeast. Yeast can produce extracellular enzymes. This study aims to evaluate the extracellular enzyme activity of yeast isolates from Gringsingan flowers (Hyptis suaveolens) in Kupang. Yeast isolation was carried out using the spread plate technique. The ability of yeast to produce extracellular enzymes was evaluated through starch and casein tests. Three types of yeast were obtained: Kodamaeaohmeri, Starmerella floricola, and Candida orthopsilosis. Isolate KB103 can produce amylase, with an amylase index of 2. Isolate Pme can produce protease enzymes, with a protease index of 3.25. Isolates KB103 and Pme were identified as Candida orthopsilosis.